Device

Part:BBa_K176226:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-13)


Repressible cell density controller: PoPS->tetR-LVA->K176096


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 855
    Illegal NheI site found at 878
    Illegal NheI site found at 1704
    Illegal NheI site found at 1727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3171
    Illegal BsaI.rc site found at 2682


Design Notes

PoPS->tetR-LVA+pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

Input1: PoPS

Input2: AHL

Output1: AHL

Output2: Cell Density

Design:

Input1 PoPS generates TetR-LVA.

pCon 0.15(K176008) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.

pCon 0.70(J23101) generates LuxR.

pLux/Tet(K176000) generates lacZalpha-ccdB (weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.


Source

K176006 K176096

References